Thiamine HCl

Thiamine hydrochloride (HCl) is a water-soluble vitamin that’s added to tissue culture growth media to provide cells with vitamin B1. It helps cells develop healthily by participating in energy production and enzymatic reactions.

Here are some ways thiamine hydrochloride is used in tissue culture:

• Hv-Ca medium

Thiamine hydrochloride is a component of the vitamin-concentrated stock solution used to culture Haloferax volcanii.

• Tris-acetate-phosphate (TAP) medium

Thiamine hydrochloride is a component of the TAP medium used to culture Chlamydomonas nivalis.

• Modified Bold 3N medium

Thiamine hydrochloride is a component of the modified Bold 3N medium used to culture Chlorella minutissima.

• Sweetpotato propagation

Thiamine improves the in vitro propagation of sweetpotato.

Thiamine is also used in the fluorometric determination of mercury